Support Services for Polyclonal and Monoclonal Antibodies
LAMPIRE has provided quality antibody support services since 1977. From antigen design and peptide synthesis to antibody purification and labeling, our expert staff is available to assist you with every phase of antibody development. The success of your antibody project is very important to us. As such, we encourage you to take advantage of our free Antigen Design and Protocol Review described below. To begin discussion about any of these services, please contact us today by email or at 215-795-2838.
Antigen Design and Protocol Review
Prior to beginning any monoclonal or polyclonal development project, you have the option of meeting with LAMPIRE's scientific staff for consultation on antigen design and immunization protocol. Using computer-aided technology, LAMPIRE scientists will analyze peptide sequences relevant to your target antigen and review with you the predicted immunogenicity. This service is provided free of charge and aids us in selecting the appropriate immunization protocol for your antigen. While we cannot guarantee every peptide sequence will generate an immune response in the host animal, this service has proven very successful in maximizing the success of each antibody development project.
| Pricing Schedule |
| Antigen Design Analysis |
No Charge |
Antigen Conjugation
To improve immunogenicity, certain antigens may require coupling to a larger molecule. These antigens may be coupled to KLH, BSA, or other requested proteins. Coupling methodologies include EDC and Glutaraldehyde.
| Pricing Schedule |
| KLH Conjugation |
Inquire |
| BSA Conjugation |
Inquire |
| Alkaline Phosphatase Conjugation |
Inquire |
| Horseradish Peroxidase Conjugation |
Inquire |
| FITC Conjugation |
Inquire |
| Biotin Conjugation |
Inquire |
| Colloidal Gold Conjugation |
Inquire |
ELISA Testing
ELISA testing can be useful in measuring and tracking the concentration of antibody in culture supernatants, bleeds, or purified materials.
| Pricing Schedule |
| Plate Charge(up to 4 samples) |
Inquire |
Note: Cost per project will vary based on the frequency of testing and type of ELISA. Also, additional antigen may be required.
Antibody Characterization
LAMPIRE offers several services to help evaluate the purity, concentration, and specificity of your antibody. Please contact our Customer Support Staff for details of these services.
| Pricing Schedule |
| BCA Total Protein Assay |
Inquire |
| Bradford Total Protein Assay |
Inquire |
| ELISA Titer Screen |
Inquire |
| HPLC |
Inquire |
| Immunoelectrophoresis |
Inquire |
| Radial Immunodiffusion |
Inquire |
| SDS-PAGE Reduced |
Inquire |
| SDS-PAGE Non-Reduced |
Inquire |
| Western Blot |
Inquire |
Antibody Purification
LAMPIRE offers a variety of purification services to complement your antibody project. From salt fractionation to antigen-specific affinity chromatography, our expert staff will purify your antibody by any of the methods below.
| Purification Services |
| Delipidation |
≤ 1 liter: Inquire
|
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> 1 liter: Inquire |
| Ammonium Sulfate Fractionation |
≤ 1 liter: Inquire
|
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> 1 liter: Inquire |
| DEAE Chromatography |
≤ 25 ml: Inquire
|
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> 25 ml: Inquire |
| IgY Purification |
Purification of first dozen eggs: Inquire |
|
Each additional dozen eggs: Inquire |
| Immunoaffinity Chromatography |
≤50mL: Inquire |
|
>50mL to ≤100mL: Inquire |
|
>100mL to ≤1000mL: Inquire |
| Protein A Chromatography: PBS w or w/o azide |
≤25mL |
>25mL
to
≤50mL |
>50mL
to
≤100mL |
>100mL
to
≤300mL |
>300mL
to
≤500mL |
>500mL
to
≤1000mL |
| Ascites |
Inquire |
Inquire |
Inquire |
Inquire |
Inquire |
Inquire |
| Monoclonal Supernatant |
Inquire |
Inquire |
Inquire |
Inquire |
Inquire |
Inquire |
| Rabbit Serum |
Inquire |
Inquire |
Inquire |
Inquire |
Inquire |
Inquire |
|
| Protein G Chromatography: PBS w or w/o azide |
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| Ascites |
Inquire |
Inquire |
Inquire |
Inquire |
Inquire |
Inquire |
| Monoclonal Supernatant |
Inquire |
Inquire |
Inquire |
Inquire |
Inquire |
Inquire |
| Rabbit Serum |
Inquire |
Inquire |
Inquire |
Inquire |
Inquire |
Inquire |
| Goat Serum |
Inquire |
Inquire |
Inquire |
Inquire |
Inquire |
Inquire |
If a dedicated column is required, please inquire for pricing.
Antibody Labeling
Purified antibodies can be labeled for use in various applications. Consultation with our scientific staff will assist you in selecting the reporter group and coupling chemistry best suited for your applications. Please inquire for pricing.
| Reporter Group |
Alkaline Phosphatase |
|
Biotin |
|
Colloidal Gold |
|
Fluorescein Isothiocyanate (FITC) |
|
Horseradish Peroxidase (HRP) |
Egg Yolk Processing
Chicken immunoglobulin yield quickly declines unless yolks are stored in buffer. LAMPIRE offers the additional service of separating the yolks and storing them in buffer. Yolks treated as such can then be shipped to the client for long-term storage at 2-8°C.
| Pricing Schedule |
| Processing of One Dozen Yolks |
Inquire |