CLASSIC-LINE Services

The CLASSIC-LINE Services are designed to optimize antibody production against small, non-immunogenic molecules such as haptens. The Basic Service yields antibody in 98 days and the Extended Service allows you to continue your project as long as needed to obtain the amount of antibody desired. To begin discussion about your project, please contact us today by email or at 215-795-2838.


Basic Service

Day Procedure
1 Pre-bleed. 2 ml serum per animal. Pre-immune serum shipped with first test bleed. Inject 0.5 ml antigen mixed with 0.5 ml Complete Freund's adjuvant.
7 Inject 0.5 ml antigen mixed with 0.5 ml Incomplete Freund's adjuvant.
14 Inject 0.5 ml antigen mixed with 0.5 ml Incomplete Freund's adjuvant.
15-27 Rest.
28 Inject 0.5 ml antigen mixed with 0.5 ml Incomplete Freund's adjuvant.
29-41 Rest.
42 Test bleed. Chickens: Daily egg collection begins.
43-55 Rest.
56 Inject 0.5 ml antigen mixed with 0.5 ml Incomplete Freund's adjuvant.
57-69 Rest.
70 Test bleed. [Note: A production bleed may be scheduled here if the animal has achieved sufficient titer.]
71-83 Rest.
84 Inject 0.5 ml antigen mixed with 0.5 ml Incomplete Freund's adjuvant.
85-97 Rabbits, Goats, Sheep, Donkeys, Llamas: Rest.
98 Rabbits, Goats, Sheep, Donkeys, Llamas: Production bleed. Chickens: Egg collection. Project terminated.


Extended Service

The 98-day Basic protocol, then (Note: Requires additional antigen)
Week Procedure
A Rabbits, Goats, Sheep, Donkeys, Llamas: Production bleed. Chickens: Test bleed. Daily egg collection continues.
B Rest.
C Injection. Boost 0.5 ml antigen mixed with 0.5 ml Incomplete Freund's adjuvant.
D Rest.

Thereafter, repeat monthly rotation as above.
Please contact our Customer Support Staff at lampire@lampire.com or 215.795.2838 for a detailed quote for your custom project. Some or all of the following forms are required to get your Polyclonal Antibody project started. Our Customer Support Staff will help determine which forms you will need.
Conjugation Questionnaire
Polyclonal Project Initiation Form