HARMONY-LINE Services
The HARMONY-LINE Services combine peptide synthesis and polyclonal antibody development into a low package price. The ECONOMY Service is the most cost-effective program, using a >75% pure peptide and yields antibody in just 50 days. The prices listed for the Basic and Extended Services described below involve a >85% pure peptide, but each of these services may substitute a >75% pure peptide upon your request.
Add an ELISA, western blot, purification, or conjugation to your project. Check our our Antibody Support Services.
Economy Service
| >75% Pure Peptide (up to 20 a.a.) |
Rapid, 50-Day Protocol |
| Conjugation to KLH |
One Production Bleed per Animal (Egg Collection for Chickens) |
| Day |
|
Procedure |
| 1 |
|
Pre-bleed. 2 ml serum per animal. Pre-immune serum shipped with first test bleed. Inject 0.5 ml antigen mixed with 0.5 ml Complete Freund's adjuvant. |
| 21 |
|
Inject 0.5 ml antigen mixed with 0.5 ml Incomplete Freund's adjuvant. |
| 30 |
|
Test bleed. Sample shipped for titer testing/screening. |
| 42 |
|
Inject 0.5 ml antigen mixed with 0.5 ml Incomplete Freund's adjuvant.
43-50 Chickens: Eggs collected. |
| 50 |
|
Rabbits, Goats, Sheep: Production bleed. |
Basic Service
| >85% Pure Peptide (up to 20 a.a.) |
Rapid, 50-Day Protocol |
| Conjugation to KLH |
One Production Bleed per Animal (Egg Collection for Chickens) |
| Day |
|
Procedure |
| 1 |
|
Pre-bleed. 2 ml serum per animal. Pre-immune serum shipped with first test bleed. Inject 0.5 ml antigen mixed with 0.5 ml Complete Freund's adjuvant. |
| 21 |
|
Inject 0.5 ml antigen mixed with 0.5 ml Incomplete Freund's adjuvant. |
| 30 |
|
Test bleed. Sample shipped for titer testing/screening. |
| 42 |
|
Inject 0.5 ml antigen mixed with 0.5 ml Incomplete Freund's adjuvant.
43-50 Chickens: Eggs collected. |
| 50 |
|
Rabbits, Goats, Sheep: Production bleed. |
Plus Service
Add an Exsanguination Bleed to either the Economy or Basic Services
| Day |
|
Procedure |
| 51-56 |
|
Chickens: Daily egg collection. |
| 57 |
|
Rabbits, Goats, Sheep: Exsanguination bleed. Rabbits, Goats, Sheep, Chickens: Project terminated |
Extended Service
The above 50-day Basic protocol, plus the services below. (Additional antigen may be required.)
| Week |
|
Procedure |
| A |
|
Rest.
|
| B |
|
Injection. Boost 0.5 ml antigen mixed with 0.5 ml Incomplete FreundŐs Adjuvant.
|
| C |
|
Rest.
|
| D |
|
Rabbits, Goats, Sheep: Production bleed. Chickens: Daily egg collection. |
| Thereafter, repeat monthly rotation as above. |