LAMPIRE's Monoclonal Department offers high-quality hybridoma development in hamsters at a very affordable price. Designed to meet the needs of both industry and academic scientists, each project begins with a FREE Antigen Design and a series of Project Scope & Definition Meetings. Projects are divided into three phases, each with its own billing cycle to spread your costs out over the course of the project. A description of each phase is presented below.
Why Hamsters
Evolutionarily distant from both mouse and rat, both types of hamsters are an excellent host for generating monoclonal antibodies to murine antigens. Differentiation of the strains is an important consideration prior to undertaking any pre-clinical or d...Read more
LAMPIRE's Monoclonal Department offers high-quality hybridoma development in hamsters at a very affordable price. Designed to meet the needs of both industry and academic scientists, each project begins with a FREE Antigen Design and a series of Project Scope & Definition Meetings. Projects are divided into three phases, each with its own billing cycle to spread your costs out over the course of the project. A description of each phase is presented below.
Why Hamsters
Evolutionarily distant from both mouse and rat, both types of hamsters are an excellent host for generating monoclonal antibodies to murine antigens. Differentiation of the strains is an important consideration prior to undertaking any pre-clinical or development service. Both models are excellent for long term stabilization of monoclonals using either strain.
Chinese Hamsters
• Creation: Approximately 70% of recombinant proteins are produced in Chinese
hamster ovary (CHO) cells
• Pharmacokinetic: Historically used to create CHO cell lines, this model has again
emerged as an ideal candidate for PK studies of the SARS-COV-2 Infection
• Production: Lampire also can assist in creating a serum-free cell line of CHO
Recombinant Therapeutic Proteins through adaptation with our custom media as
a fee for service, or to provide you with the media for in-house production
Armenian Hamsters
• Development: Armenian Hamsters elicit antibody responses to a broad repertoire of
antigens, which are demonstratively different to those generated in mouse or rat.
• Stability: Hamster monoclonals are easily purified by Protein A affinity
chromatography and have been successfully used in multiple applications, including
development of high affinity neutralizing reagents to murine antibodies, cytokines,
receptors, and transcription factors.
• Quick responders; Often a fusion candidate is presented in 30 Days
Free Antigen Review
Please take advantage of our FREE Antigen Design and Protocol Review. Without obligation, we will set-up a meeting for you with our scientific staff to discuss your project. We will provide you with an estimate of project feasibility, animal model, timelines and costs. To begin your discussion, please contact projman@lampire.com or call 215-795-2838.
Phase I: Project Design and Immunization
Each project begins with a Project Scope meeting between you and our technical staff in an effort to assure the success of your project. Upon antigen arrival, animals are then immunized, according to the approved protocol and titer is monitored by ELISA until sufficient titer is reached to warrant fusion between spleen cells and an immortal, non-secreting myeloma cell line. Note: Any subsequent injections and test bleeds, if required, will be performed only after consultation with our technical staff.
Phase II: Cell Fusion
After candidate animals are selected, the number of required fusion plates is determined, and the fusions are performed. Fusion plates are screened by ELISA to identify hybrid cell lines that produce antigen specific antibody. Several fusions may be required to obtain a hybrid cell line that produces the desired antibody. Fusion candidates will be approved by the client before the fusions are performed and charges are incurred.
Phase III: Cloning
Positive cell lines from Phase II will be expanded to produce sufficient culture to preserve the parent cell line and to subclone the identified positives by limiting dilution. Typically 3 cell lines are chosen for Phase III, but this number can be increased based on client requirements. The expansion culture of the selected positive cell lines will be preserved in liquid nitrogen. Up to fifteen (15) additional positive cell lines may be cryopreserved individually at LAMPIRE or up to 100 may be cryopreserved as pools. These cell lines may also be shipped to you, if requested. Recloning of selected positives will be performed as needed to achieve antibody production stability. Clones must exhibit >95% antigen-specific antibody production to be considered stable.
Get Started!
Some or all of the following forms are required to get your Monoclonal Antibody project started. Please contact our Project Management Team at projman@lampire.com or call 215.795.2838 to help determine which forms you need or for assistance filling them out.
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